Figure 1. A) General organization of SapTrap-assembled constructs. Italics indicate the fragments that are assembled to build the final construct, and blue upper-case letters are the 3-nucleotide overlaps that enable ordered assembly. B) Efficiency of construct assembly (% correct clones) using SapTrap (Schwartz and Jorgensen 2016) or a ccdB/Gibson-based approach (Dickinson et al. 2015).