Figure 1 Expression patterns of
mls-2p:GFP::
mls-2 transgene, mNG::SEC::
mls-2 knock-in, and mNG::
mls-2 knock-in:(A) Structure of the
mls-2 locus,
mls-2p::GFP::
mls-2 transgene (Jiang et al., 2005), mNG::SEC::
mls-2 knock-in, and mNG::
mls-2 knock-in. mNG, mNeonGreen. SEC, self-excising cassette containing transcriptional terminators, a dominant roller phenotype marker
sqt-1(
e1350), Cre driven by a heat shock promoter, and a hygromycin resistance gene. The SEC cassette is flanked by LoxP sites.(B-H) Representative images of GFP::MLS-2 expression from a
mls-2p::GFP::
mls-2 extrachromosomal transgene in a first-stage larva (B), mNG expression from mNG::SEC::
mls-2 knock-in in a first-stage larva (C), and mNG::MLS-2 expression from mNG::
mls-2 knock-in animals in different developmental stages (E-H). Integrated transgenes of
hlh-16::H1-wCherry and
odr-1p::TagRFP (or
odr-1p::DsRed) were used as early and late AWC markers, respectively. Insets in panels B, C, E, F, and G are magnified by 2-fold. Scale bar, 10 um. Anterior to the left and ventral to the bottom in lateral or ventrolateral views of the head region in B, C, and F-H; ventral view in E. (D) Expression of the AWCON marker
str-2p::TagRFP from an integrated transgene in wild type and mNG::
mls-2 knock-in animals. n, total number of animals scored.