Fig. 2. Constitutive expression of
numr-1 and
numr-2. (Upper panel) Superimposed fluorescence and DIC images of JF86(mtEx61) C. elegans at four larval stages (L1, L2, L3 and L4) and adults. The insert in the adult panel shows transgene expression in vulval muscles. Coexpression of
numr-1 and
numr-2 is denoted by yellow fluorescence. The arrowheads indicate the location of the pharyngeal grinder in each nematode. (Lower left panel) qRT- PCR analysis of
numr-1/-2 steady-state mRNA levels at different developmental stages. Total RNA was isolated from embryos (emb), L1, L2, L3, L4 larvae, and adult C. elegans. All measurements were normalized to the mRNA level of
rbp-12 and the fold change was relative to the steady-state level mRNA measured in adult nematodes. Values are mean
log2fold change ± s.e.m. (
n3). Asterisks indicate statistically significant difference from adult nematodes (P<0.0001). (Lower right panel) The head region of JF85(mtEx60) showing
numr-1 expression and DiI-stained neurons.
numr-1 expression (green) does not coincide with the ciliated amphid neurons (red). The position of the grinder is indicated.