Picture from Squirrell JM et al. (2006) Mol Biol Cell "CAR-1, a protein that localizes with the mRNA decapping component DCAP-1, is ...."
Figure 3. CAR-1 localizes to cytoplasmic puncta and can associate with the anaphase spindle. (A) Columns indicate the following developmental stages: pronuclear migration, 2-cell interphase, and 4-cell interphase. The top row is a multiphoton time sequence of a living embryo showing changes in distribution of GFP::CAR-1, with few small cytoplasmic puncta (small arrowhead) present during pronuclear migration and larger puncta developing in the posterior during and after cytokinesis (arrow). There was an increase in the number of small cytoplasmic puncta during and after the 2-4 cell division; midpronuclear migration: 0.007 ± 0.004 puncta/u2, N = 4 embryos; 2-cell interphase: 0.004 ± 0.003 puncta/u2,N = 8 embryos; AB furrow initiation: 0.013 ± 0.004 puncta/u2, N = 8 embryos; interphase Aba = 0.038 ± 0.007. An antibody to CAR-1 showed a similar pattern in fixed embryos (bottom row). (B) Confocal images of mouse embryonic stem cells (ES) or fibroblasts (STO) transfected with either GFP only or with the full-length mCAR-1 tagged with GFP. The mCAR-1::GFP was expressed in numerous discrete foci in both embryonic stem cells and in fibro-blasts. (C) Multiphoton time series of living embryos showing CAR-1 spindle localization. GFP::CAR-1 was initially absent from the spindle and then accumulated on the spindle region, and as the spindle elongated, the CAR-1 label extended along the spindle to include the pericentriolar region. Lower series shows an enlargement of the spindle region at 45-s intervals, with the final image compared with the organization of the ER (lowest image shows GFP::SP12). These images were taken with higher detector gain than those in A to accentuate the nonpunctate cytoplasmic distribution of GFP::CAR-1. Scale bars, 10 μm.