(A) Photograph of the head of a transgenic animal expressing GFP under the control the promoter of
delm-2(Pdelm-2::GFP) (front is to the left). (B) same as in (A) for a
delm-1 mutant expressing AgTRPA1 under the control of Pdelm-2 (
delm-1;Pdelm-2::AgTRPA1;Pdelm-2::GFP).
delm-2 is expressed in OLQ and IL socket glia (Han et al., 2013). (C) Schematic representation of a OLQ or IL socket glia in the indicated genetic backgrounds. In animals expressing AgTRPA1 in socket glia, influx of cations is present only at temperatures above 27°C due to the temperature sensitivity of AgTRPA1. WT is N2. (D) Ratios of responses (responses/total number of trials, labelled on the Y axis as "responses/tot trials") assayed at 22°C for wild type animals (N2, 0.770 ± 0.042, n = 20),
trpa-1(
ok999) (0.210 ± 0.042, n = 20),
delm-1(
ok1226) (0.250 ± 0.035, n = 20), and
delm-1 expressing mosquito TRPA1 (AgTRPA1) in OLQ and IL1 socket glia (
delm-1(
ok1226);Pdelm-2::AgTRPA1, 0.270 ± 0.036, n = 20). (E) Ratios of responses assayed at 28C for wild type animals (N2, 0.6480 ± 0.026, n = 50),
trpa-1(
ok999) (0.228 ± 0.024, n = 50),
delm-1(
ok1226) (0.248 ± 0.025, n = 50), and
delm-1 expressing mosquito TRPA1 (AgTRPA1) in OLQ and IL1 socket glia (
delm-1(
ok1226);Pdelm-2::AgTRPA1, 0.624 ± 0.025, n = 50). Data are expressed as means ± SE, ****p<0.0001 by ANOVA with Bonferroni correction.