Figure 4. Transcriptional control of
daf-9 by
daf-12. (A,B) An N2 wild-type larva carrying a
daf-9p::
daf-9 genomic::GFP transgene at late L2 stage. (A) Normaski image. (B) Fluorescence image showing strong
daf-9::GFP expression in the hypodermis. (C,D) A
dpy-7(
sc27)
daf-12(
m20) larva carrying a
daf-9p::
daf-9 genomic::GFP transgene at late L2 stage. (C) Normaski image. (D) Fluorescence image showing
daf-9::GFP expression in the XXXL/R cell but not in the hypodermis. (E) Summary of
daf-9::GFP expression pattern driven by five transgenes that encompass different promoter, exonic and intronic sequence from the endogenous
daf-9 locus. In construct v, a nuclear receptor (NR) consensus half site (underlined) in intron 1 was mutated to a LexA-binding site with the altered bases in grey. Transgenes i, ii and iv rescued the dauer arrest phenotype of
daf-9(
e1406)
dpy-7(
sc27) mutant animals. n.d., not determined.