Figure 2.
gar-3 encodes a muscarinic acetylcholine receptor expressed in neurons and in the pharyngeal muscle. (A) Alignment of the N-terminal portion of the GAR-3 amino acid sequence with human muscarinic receptors (the GAR-3 sequence data are available in the GenBank databases under accession no. AF139093). The
vu78 point mutation is indicated by an arrowhead. The
lg1201 deletion is underlined. The deletion also disrupts the intron-exon junctions identified by Hwang et al. (1999) so that sequences 3' to the breakpoints are likely mistranslated. A complete alignment is available in the supplementary information at
http://www.genetics.org/supplemental/. (B) A
gar-3 reporter is expressed in neurons of the extrapharyngeal nervous system; in the pharyngeal muscles of the metacorpus, isthmus, and terminal bulb; and in pharyngeal neuron I3 (obscured by muscle staining). Bar, 50 um.