drag-1 is expressed and functions in the same cell types as the Sma/Mab pathway components. (A-L) Images shown are side views with anterior to the left and posterior to the right.
drag-1p::gfp (A-D) and
sma-6p::rfp (E-H) colocalize in pharyngeal (A,E,I), hypodermal (B,F,J) and intestinal (C,G,K) cells, but do not colocalize in the male tail (D,H,L). I-L are merged images of A-D and E-H, respectively. (M-O) DRAG-1::GFP (M,N) or LIN-12TM::GFP (O; see Fig. 5 for details) localization in pharyngeal (M), hypodermal (N) and intestinal (O) cells, as visualized by anti-GFP antibody staining. Note that the GFP signal (green) is located outside of the nucleus (blue, stained with DAPI), both at the cell surface and inside the cell. Arrow heads, hypodermal cells; arrow, pharynx. (Q-U) The M lineage expression pattern of
drag-1 using LIN-12TM::GFP (green; see Fig. 5 for details). Anti-FOZI-1 antibody staining (red) was used to mark M lineage cells from the 4-M to the 12-M stage (S-U). Only one focal plane was shown for the 8-M (T) and 12-M (U) stage worms. DRAG-1::GFP is present from the 1-M to the 4-M stage (O-S), then becomes fainter at the 8-M stage (T) and undetectable after the 8-M stage (U).