Figure 1. CID-1 regulates stress resistance. (A) Longitudinal thermotolerance assay of
cid-1(
rf34::Tc4) mutant lines. (+/+) indicates wild-type
cid-1 gene. (B) Longitudinal thermotolerance assay of
rrf-3(
pk1426)
cid-1(RNAi) worms. The
rrf-3(
pk1426) mutation renders worms more sensitive to RNAi. The complete thermotolerance data can be found in table S1. (C) HU-resistance assay. (Left) Wild-type N2 worms showed arrested development at the L1/L2 stage when eggs were placed on HU plates. (Right)
cid-1(
rf34::Tc4) mutants develop into fertile adults at the same concentration of HU. (D) Life span of
rrf-3(
pk1426)
cid-1(RNAi) worms at 20C. (E) Transgenic worms expressing a CID-1::GFP gene fusion. (Top) Composite fluorescent and bright-field micrographs and corresponding fluorescent micrographs of the fusion protein localized to the first layer of intestinal cells (Int 1) and to posterior intestinal cells. (Bottom) A high-resolution fluorescent micrograph of the fusion protein localized to the cytoplasm of the posterior intestinal cells.