Figure 6. HIM-5 is enriched on the autosomes. (A and B) Anti-HIM-5 staining in wild-type germlines. (A) HIM-5 can be seen at low levels in the mitotic zone, accumulates in the transition zone and early pachytene, and decreases mildly upon entry into pachytene and more dramatically in late pachytene. Costaining with anti-XND-1 reveals differences in the accumulation patterns of these two proteins. (B) Enlarged region of late pachytene shows the overlap between XND-1 (green), HIM-5 (magenta), and DNA (blue) showing their exclusion from the X chromosome (orange arrowheads). Similar enrichment is observed in mitotic and early pachytene nuclei (not shown). Bar, 2 um. (C and D) Localization of HIM-5 is impaired in xnd-1
(C) and him-17
(D) mutants. Dissected gonads were colabeled with DAPI, anti-HIM-5, anti-XND-1, and as a positive control for staining, nuclear pore marker anti-mAb414.