Figure 1. UNC-108 is required for stress-induced sleep (SIS) and acts downstream of EGF signaling. (A,B) Compared to wild-type N2,
unc-108(lf) mutants are defective for SIS induced by Cry5B toxin or UV light (P< 0.0001, one-way ANOVA with Dunnett's multiple comparisons test). Animals were exposed to Cry5B-expressing bacteria as described (Hill et al., 2014) and examined 20 min later, or exposed to UV radiation as described (Goetting et al., 2017) and examined 60 min later. These time points have been shown to be associated with robust ALA-dependent quiescence (Hill et al., 2014; Goetting et al., 2018). (C) LIN-3/EGF overexpression induces sleep in wild type but not in
unc-108(
csn2) animals (P< 0.0001, two-tailed Fisher's exact test).EGF overexpression from a hs:
lin-3 transgene (Van Buskirk and Sternberg, 2007) was induced by mild heat shock (33C for 10 min) and animals were examined 60 min later for quiescence. In all panels, quiescence was defined as a complete cessation of locomotion, head movement and pharyngeal pumping during a 5 sec examination on a stereomicroscope. Mean and SD of three independent trials are shown, and each data point represents one trial of 25 well-fed young adult animals.