FIG. 3. JP promoter assay in nematode. Adult nematodes carrying the JP-GFP or
myo-3-GFP transgene were analyzed under a confocal microscope, and fluorescence (upper panels) and bright-field images (lower panels) are shown. In the JP-GFP transgenic nematodes, body-wall muscle, vulval muscle, and pharynx muscle cells were fluorescence-positive. A similar fluorescence pattern was observed in the
myo-3-GFP nematodes, but pharynx bulb muscle cells were fluorescence-negative. Arrows indicate the vulva. Scale bars: 200 um in the whole-animal image and 50 um in the other images.