Picture from Fernandez AG et al. (2006) Curr Biol "MEL-28 is downstream of the Ran cycle and is required for nuclear-envelope ...."
Figure 1. MEL-28 Shuttles between the Nuclear Periphery and the Kinetochore. Wild-type embryos were stained with anti-MEL-28 antibodies (first column, green), monoclonal anti-nucleoporin antibody mAB414 (second column, red), and DNA (DAPI, third column, blue). Merged images are shown in the fourth column. Before the onset of mitosis (A-L), MEL-28 is present at the nuclear periphery and within the nucleus. On the nuclear periphery, MEL-28 overlaps with the interior border of the mAB414 staining ([H], inset). At metaphase (M-P), MEL-28 decorates two stripes on either side of the metaphase chromosomes, coincident with the holocentric kinetochore ([M], white arrowhead). By early anaphase (Q-T), nuclei have already reconstituted their mAB414 and MEL-28 nuclear periphery localization. As the AB cell prepares to divide (U-FF), MEL-28 diminishes from the nuclear envelope and becomes enriched on the chromatin ([U], white arrow), although the mAB414 nuclear-envelope staining is still intact ([V], yellow arrow). Polar bodies (yellow arrowheads) mark anterior. The scale bar represents 10 uM.