Figure 7. Immunocytochemical analysis of whole-mount embryos using anti-bpat- 3cyto. Embryos are oriented with the head to the left. All embryos except those shown in E and G were colabeled with the monoclonal antibody MH27, which recognizes a desmosomal component at the apical surface of hypodermal and a subset of pharyngeal cells. The pattern of MH27 staining, which forms a grid-like pattern, can be seen in H. (A) Dorsal view of staining in a comma stage embryo, showing
bpat-3 staining in developing muscle quadrants. (B) Same embryo as in A, mid-focal plane of embryo, showing
bpat-3 staining in a subset of cells comprising the pharynx, tentatively identified as the marginal cells. (C) Dorsal view of a 1.5-fold embryo,showing
bpat-3 staining in the dorsal muscle quadrants. (D) Same embryo as in C, mid-focal plane of embryo, showing staining in the pharynx, again tentatively identified as the marginal cells. (E) Three-fold embryo, showing
bpat-3 staining in the anal depressor muscle.
bpat-3 staining in body wall muscles is out of focus. Staining in the intestine is most likely independent of
bpat-3 expression as it was also detected in
pat-3(
rh54) embryos (see Fig. 5 H) (F) Threefold embryo, right side, showing Bpat-3 staining in the right side anterior coelomo- cytes. (G) Threefold embryo, showing
bpat-3 staining in a neuronal cell body and process located laterally in the anterior portion of the embryo, with the process extend- ing anteriorly. This neuron has been tentatively identified as the touch neuron ALM (see text). (H) Embryos stained with anti-
bpat-3cyto and preincubated with 10 ug/ml of the cytoplasmic peptide show no detectable staining. Embryos were colabeled with MH27 to ensure that the animals had been permeabilized; the pattern of MH27 staining is shown for comparison with the other figures. Bars: (A-F and H) 5 um; (G) 10 um.