Figure 1. A) General organization of SapTrap-assembled constructs. Italics indicate the fragments that are assembled to build the final construct, and blue upper-case letters are the 3-nucleotide overlaps that enable ordered assembly. B) Efficiency of construct assembly (% correct clones) using SapTrap (Schwartz and Jorgensen 2016) or a ccdB/Gibson-based approach (Dickinson et al. 2015).
Expression pattern of C01H6.9 (haspin; G) as determined by promoter-GFP reporter constructs. (G) C01H6.9 expression is shown in one of the phasmid neurons, PHB.
Expression of MBOA-7::GFP in an early embryo (D and E) and a second-stage larva (F and G). Nomarski micrographs (D and F) and corresponding MBOA-7::GFP expression (E and G). MBOA-7::GFP was ubiquitously expressed throughout development. Asterisks indicate the anterior of the larva (F and G). Bar, 10 um (D and E) and 200 um (F and G).
(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.
G, H, PNG-1::GFP expressed from a pan-epithelial or pan-neuronal promoter shows cytoplasmic localization. Epithelial seam cells (G) and a tail neuron (H) are shown. Scale bars, 10 um.