Figure 4. (A-C) Expression of a rescuing epitope-tagged odr-2
clone, HA#3, in the head, mid-body, and tail regions, respectively. The hemagglutinin epitope was introduced into the common region of ODR-2 and visualized by whole-mount antibody staining of adult animals. Anterior is to the left and dorsal is up. ODR-2 is expressed at high levels in axons and concentrated in the nerve ring (NR) and ventral nerve cord (VNC), although expression in other nerve bundles is also visible (L, lateral; SL, ventral sublateral; C, commissures). Only weak staining was observed in the region of sensory dendrites (D). Because ODR-2 is not present in the cell body in most cells, the neurons expressing these HA epitopes could not be identified. Expression is probably present in numerous classes of sensory neurons, interneurons, and motor neurons. Expression was not observed in AWC cell bodies. (D-F) Expression of odr-2
GFP fusion clones. GFP expression patterns in the head/nerve ring region of adult animals were collected as confocal planes and projected onto the images shown; anterior is to the left and dorsal is up. Non head region neuronal GFP expression was also observed (data not shown). D-F show GFP driven by sequences upstream of the predicted translation start sites of odr-2
(16), and odr-2
(18), respectively. Expression driven by the 2b upstream region was observed in AIZ, AIB, AVG, RIF, PVP, and RIV interneurons, SIAV motor neurons, and IL2 and ASG sensory neurons. Faint expression driven by the 16 upstream region was observed in SMD and RME motor neurons, as well as in several other neurons in a variable pattern. Expression driven by the 18 upstream region was observed in SMB and RME motor neurons, in ALN and PLN sensory neurons, and in RIG interneurons. Neuron names are denoted in red and the names of nerve bundles are denoted in white (NR, nerve ring; VNC, ventral nerve cord; SL, ventral sublateral cords; D, dendrites).