FIGURE 4. Expression of
gpa-2::lacZ and
gpa-3::lacZ in adult hermaphrodites. (A) Right lateral view of a syls7 (gpa- 2::lacZ) adult hermaphrodite. Anterior is right, dorsal up. In the tail, the interneuron PVTis stained blue (small arrowhead). Typical staining in the phasmid neurons, PHA and PHB, and in the anal sphincter muscle does not appear in this animal. Neural nuclei and processes in the pharyngeal nerve ring can be seen anteriorly (large arrowhead). (B) Ventral view of the pharyngeal region of a syls7 adult hermaphrodite. Anterior is left. The position of the pharyngeal nerve ring is revealed by stained processes indicated by a black arrow. Bilateral pairs of cells anterior and posterior to the nerve ring are indicated by black arrowheads. Neurons in the ventral ganglion are indicated by white arrows. See text for cell identifications. Typical staining in pharyngeal neurons does not appear in this animal. ( C ) Lateral view of the anterior end of an adult hermaphrodite bearing
gpa-2::lacZ lacking a nuclear localization signal. Anterior is left. The anterior ends of the left members of two pairs of processes, one each from neurons in the anterior and lateral ganglia, projecting into the left lateral lip are indicated by arrows. One of the amphid channels is indicated by an arrowhead. (D) Right lateral view of a syls28 (
gpa-3::lacZ)adult hermaphrodite. Anterior is right, dorsal up. In the tail, stained nuclei of the phasmid neurons, PHA and PHB, are indicated with small arrowheads. Nine bilateral pairs of nuclei in the pharyngeal nerve ring are indicatedby an arrowhead. (E) Left lateral view of the pharyngeal region of a syZs28 adult hermaphrodite. Anterior is left, dorsal up. Nine neural nuclei that occur in bilaterally symmetric pairs are stained, of which only some members of some pairs are visible in this picture. From anterior to posterior, the amphid neurons ASK, ADL, and AS1 are indicated by arrowheads. See text forother cell assignments. (F) Left lateral view ofthe headof an adult hermaphrodite bearing
gpa-3::lacZ lacking a nuclear localization signal. Processes projecting anteriorly from the nerve ring to the left lateral lip are stained. The amphid pore is indicated with an arrowhead. (A and D) Magnification -4OX. (B, C, E, and F ) Magnification 19OX. A-D and F stained with X-Gal; E stained with Salmon-Gal. All panels were photographed on Ektar 25 film (Kodak) with Nomarski optics.