Figure 1. TRPM channels in C. elegans A) A phylogenetic tree of C. elegans, Drosophila, and human TRPM proteins. DmTRP indicates the TRP channel of D. melanogaster. CED-11, GTL-1, GTL-2, and GON-2 are C. elegans TRPMs. TRPM 1-8 are human proteins. Relationships were inferred by the Neighbor-Joining Method (Saitou and Nei, 1987). B) Expression of
gon-2,
gtl-1, and
gtl-2. Pgon2s(9)::gfp and Pgtl1(7)::gfp fusion genes were strongly expressed in the intestine. Bar, 50 um. C) Characterization of
gtl-1 deletion mutants
tg113 and
ok375. (Top) The structure of the wild-type
gtl-1 gene and two pairs of PCR primers (ER1 and EL1, and ER2 and EL2). (Middle) The deleted region of
tg113. (Bottom) The deleted region of
ok375 (a 50 nucleotide insertion was found at the junction). All numbers shown at the junction sites are the numbers of nucleotides starting from the first ATG initiation codon.