Figure 2. GCY-28 in the AIA interneurons is important for the interaction of two signals. A, The expression of the
gcy-28.d::gfp fusion gene under control of the
gcy-28.d promoter at the adult stage.The site of expression is indicated by an arrowhead. Scale bar,10um.B,The phenotypes of
gcy-28 mutant expressing
gcy-28. dcDNA in AWCON+OFF neurons or AWCON were analyzed by the interaction assay. Worms carrying the transgene (+Ex, gray columns) and worms not carrying the transgene (-Ex, black columns) were reared on the same culture plate and subjected to the assay on the same assay plate to compare the indices. White columns indicate non transgenic worms reared on separate culture plates and subjected to the assay on separate assay plates. C-E, The phenotypes of
gcy-28 mutants expressing
gcy-28.c cDNA by various promoters were analyzed by the interaction assay. The expression of H20 promoter: pan-neuron. The expression of
ins-1 promoter: neurons including AIA. The expression of
unc-86 promoter: several neurons including AIZ. The expression of
ttx-3 promoter: AIY. The expression of
odr-2 promoter: neurons including AIB. The expression of
tax-4,
odr-10, or
osm-3 promoter: sensory neurons including ADL, ASE, ASH, AWA, and AWC.
gcy-28(
qj4 ) animals carrying phsp::
gcy-28.c were heat shocked at young adult stage. F, The schematic diagram for the putative neural network for the interaction of two sensory signals, extracted from the complete circuit (White et al., 1986). Sensory neurons are shown as triangles and interneurons as hexagons. Arrows indicate chemical synapses, and lines with T-shaped ends indicate gap junctions. The AIA neurons expressing GCY-28.d are emphasized. G,
gcy-28 mutants expressing
gcy-28 cDNA in AIA interneurons by
gcy-28.d promoter were analyzed in the interaction assay. Open bars show non transgenic animals for comparison. B-E, G, +Ex and -Ex worms were compared. Error bars in B-E and G indicate SEM. *p < 0.01. WT, Wild type.