Figure 2. GAR-2/M2 is expressed in cholinergic and GABAergic motor neurons and requires
goa-1 Gao. A, Representative image of ventral cord motor neurons expressing soluble GFP driven by the
gar-2 promoter (a) and soluble DsRed2 driven by the
unc-25 GAD promoter (b). The two images are superimposed to illustrate neurons that coexpress GFP and DsRed2 (c). Asterisks mark GABA motor neurons expressing GAR-2. YFPVenus::GAR-2 expression in a dorsal motor axon (d). Scale bar: a-c, 10 um; d, 3.3 um. B, Paralysis time course in 1 mm aldicarb for wild type (open black circles),
gar-2 (filled green squares), rescue:cDNA rescue under the
gar-2 promoter (filled red circles),
gar-2(GABA):cDNA rescue under the
unc-25 GAD promoter (open purple squares), and
gar-2(ACh):cDNA rescue under the
unc-17 VAChT promoter (filled blue triangles). C, Paralysis time course in 1 mm aldicarb for wild type (filled black circles),
goa-1 Gao (filled red circles),
gar-2(ACh):cDNA rescue under the
unc-17 VAChT promoter (filled blue circles), and
goa-1;
gar-2(ACh) (filled green circles). Data are mean +/- SEM.