Figure 4.
fox-1 expression analysis. (A) A Western blot with extracts collected from a pure population of L4/adult males (M) and N2 animals at the different stages of development from embryo (E), L1, L2, L3, L4, to young adult (A), was probed with affinity-purified rabbit polyclonal antibody against bacterially expressed FOX-1. The Ponceau-stained blot is shown below for loading control. The position of the 45- kD FOX-1-specific band is indicated to the left. (B)
fox-1::lacZ expression in adult XX and XO animals is confined to small subsets of cells in the head and the tail. The expression pattern in XXs is distinct from that in XOs.