Figure 7. Experimental Validation of the Model's Predictions (A,B) As examples for the time-course analysis, a mid-L2 larva at +22 h (A-A'') and a late L2 larva at +28 h (B) are shown. For each animal, the Nomarski, CFP ('), and YFP ('') channels are shown. (C-C'') Example of a
lin-15(
n309) larva at the late L2 stage showing simultaneous expression of EGL-17::CFP and LIP-1::YFP in P7.p, P5.p, and P4.p. All images were taken with identical camera and microscope settings. Scale bar in C is 10 um. (D) Quantification of the EGL-17::CFP (blue dots) and LIP-1::YFP (orange dots) signals in P5.p, P6.p, and P7.p of ten to 12 animals for each time point. The relative fluorescence intensities are shown as percent values of the maximal EGL-17::CFP and LIP-1::YFP signals, respectively, observed during the time-course analysis. (E,F) Semiquantitative representation of the EGL-17::CFP and LIP-1::YFP expression patterns observed in the VPCs of wild-type (E) and
lin-15(
n309) (F) late L2 larvae. Signal intensities were classified as indicated by the color legend on the right.