Picture from Cueva JG et al. (2007) J Neurosci "Nanoscale organization of the MEC-4 DEG/ENaC sensory mechanotransduction ...."
Figure 3. Nanoscale position of the MEC-2 and MEC-4 MeT channel subunits and MEC-5 collagen in wild-type TRNs. A , Schematic cross-section showing the relative positions of the cuticle (gray), four muscle quadrants (green), hypodermis (brown), PLM (green), PLN (blue), and ECM (purple). Structures not to scale. Adapted from WormAtlas (Altun and Hall, 2005). B , Electron micrograph showing a cross-section through the cuticle (gray), ECM (purple), PLM (blue), and PLN (green). Cuticle (apical) side is up. Scale bar, 200 nm. C , Immunoelectron micrographs of thin sections labeled with anti-MEC-2 (top), anti-MEC-4 (middle), and anti-MEC-5 (bottom). Insets are digitally magnified (2×) views of each label cluster. Filled arrowheads indicate gold beads; open arrowhead indicates a mitochondrion in PLM. Scale bar, 200 nm. D , Circumferential position of MEC-2 (top), MEC-4 (middle), and MEC-5 (bottom). Symbol size is proportional to the number of gold beads in a cluster in each section; the smallest symbol corresponds to a single gold bead and the largest symbol corresponds to four gold beads. The average ECM density for the labeled sections is shown as a grayscale annulus around the neurite cross-section. E , MEC-5 collagen but not MeT channel subunits exhibit an apical bias. *p < 0.01, χ2 test. The number of labels in each category is indicated in parentheses.
Picture from Ao W et al. (2004) Science "Environmentally induced foregut remodeling by PHA-4/FoxA and ...."
Figure 1. Five pharyngeal clusters. Pie charts of protein categories for all candidate pharyngeal genes (A) and individual clusters (B to E). GST, glutathione S-transferase; ECM, extracellular matrix protein; Prot. Inh., protease inhibitor; ShTK, ShTK domain-containing protein; Chan./Recep., channel molecule or receptor; Cytosk./Musc., cytoskeleton or muscle protein; Enzyme, metabolism enzyme; DUF139, domain of unknown function 139 domain; Trnx Factor, transcription factor; and Kin./Phos., kinase or phosphatase. (F to J) Previously uncharacterized genes were expressed as predicted. C49G7.4 in Ph-G, confirmed by co-staining with a gland-specific antibody, J126 (19), M03D4.4 in Ph-M, F21D5.9 in embryonic Epi, ZK1067.7 in late muscles and marginal cells from Topo Mounts 14 or 29 (Ph-MMC) (9), and C15H9.9 in Ph-MC. Dotted lines indicate the pharynx [(F) to (J)].