Picture from Ung, Hailey M. et al. (2020) MicroPubl Biol
Genetic modifiers of huntingtin protein aggregation in C. elegans.: A) Representative fluorescent confocal micrographs of a parental strain (EAK103) expressing Htt513(Q128)::YFP in body wall muscle cells, or of three independent EMS-derived mutants referred to herein as modifiers of Htt aggregation (mha). B) Quantification of the number of Htt513(Q128)::YFP protein aggregates in each of the mha mutants compared to both the parental strain and to strain EAK102, which expresses the protein Htt513(Q15)::YFP as a negative control for aggregation. Mean aggregate number for at least 50 individuals is shown for each indicated time point. Animals were sacrificed at the time of scoring; therefore each day represents a completely independent experiment. Error bars represent standard error of the mean. Student t-tests were performed, comparing aggregation in each mha strain to that of the parental strain. Because no statistically significant differences were observed over time, data for each of the three days were pooled for the purpose of further statistical analyses. The symbol *** denotes a p-value less than 0.0001. C) Representative western blot of Htt513(Q128)::YFP protein detected with an anti-polyQ antibody or α-tubulin as a loading control. N2 and YFP-expressing animals (AM134) are shown as negative controls. D) Western blot quantification using Image J analysis software. Mean Htt protein levels are shown for three biological replicates, error bars represent standard error of the mean, and the p-value is the result of a student t-test.