Figure 3. Localization of CLE-1 and type IV collagen in wild-type and
cg120 animals. Immunofluorescent localization of CLE-1 (A-H) and type IV collagen (I and J) are shown in wild-type (A-D and I) and
cg120 (E-H and J) animals. In all panels, anterior is left, and dorsal is up. (A) A fourfold embryo shows strong CLE-1 staining associated with the nerve ring (arrowhead). (B) In larval animals, CLE-1 accumulates on the nerve ring (arrowhead) and the dorsal (dc) and ventral (vc) nerve cords. (C) In adults, staining for CLE-1 is strong on the nerve ring and nerve cords (dc and vc) and is weakly detected on the surfaces of the pharynx (p), intestine (i), and gonad (g). (D) Under body wall muscle of an adult, CLE-1 accumulates strongly at the junctions between muscle cells (arrowheads) and along muscle-dense body lines (small arrows) but is not coincident with the dense bodies. Staining on the ventral nerve cord is distinctly punctate. (E) Weak CLE-1 accumulation on the nerve ring of a
cg120 embryo. (F) CLE-1 localization to the nerve ring (arrowhead) and dorsal (dc) and ventral (vc) nerve cords of a
cg120 larva. (G) Accumulation on nerve ring (arrowhead), nerve cords (dc and vc), pharynx (p), and intestine (i) of a
cg120 adult. (H) Under the body wall muscle of a
cg120 adult, CLE-1 accumulates along dense body lines (small arrows) and along the ventral nerve cord (vc) as in wild type. However, there is no accumulation at the junctions between muscle cells (arrowheads), and the pattern on the nerve cord is more diffuse. (I and J) Wild-type and
cg120 L1 larvae show type IV collagen localization in the basement membranes of the pharynx (p), intestine (i), and gonad primordium (g).