Figure 6. Gene Expression in
cyd-1(
q626) Mutants (A-H) (A-C) Wild-type L1 larvae raised at 25C; (D-F)
cyd-1(
q626) L1 larvae raised at 25C. Solid arrow, SGPs expressing marker; open arrowheads, SGPs that do not express marker; white arrow, nongonadal cell-expressing marker. All images are XX larvae, but the same results are seen in XO larvae. (A-C) Wild-type SGPs express (A)
fkh-6::GFP, (B)
lag-2::GFP, and (C) rnr::GFP. (D) Some
cyd1(q626) SGPs express
fkh-6::GFP (arrow), and others do not (open arrowhead). (E) All
cyd-1(
q626) SGPs express
lag-2::GFP. (F) Most
cyd-1(
q626) SGPs do not express rnr::GFP (open arrowheads), but nongonadal cells express rnr::GFP normally (large arrowhead). (G)
gon-4(RNAi) gonads express
fkh-6::GFP. (H) Time course of
fkh6::GFP expression in WT (black),
cyd-1(
q626) (red),
elf-1(RNAi) (blue), and
cyd-1(
q626);
efl-1(RNAi) (purple) gonads.