Figure 3. Decreasing Expression of
lin-28:GFP after the L1 Stage. Fluorescence micrographs of animals carrying the
lin-28:GFP transgene (VT800) are shown with a DIC image of the same field at each of the four larval stages. For each stage, a different animal is shown; each is representative of VT800 at the indicated stage. Fluorescence is greatest in the L1, is detectable but diminished in the L2, and is only autofluorescence later. In the L1 animal, cells of various types express
lin-28:GFP, including hypodermal seam cells (se), body muscle (bm), and neurons (n).