Figure 4. CEH-17 and CEH-14 contribute to
deg-3 expression in ALA. (A) ALA neuron survival at the L1-L3 stages in animals carrying the
deg-3(gf) mutation. The
ceh-14 null mutation suppresses the ALA degeneration phenotype of
deg-3(gf), suggesting that CEH-14 contributes to early
deg-3 expression in ALA. *P<0.01 versus
deg-3(gf), Fisher's exact test. (B) Percentage of animals expressing a
deg-3 reporter gene,
des-2:GFP, in ALA at the first and last larval stages.
des-2:GFP expression in ALA is dependent on
ceh-14 across larval stages but dependent on
ceh-17 only at the later stage. ° no significant difference and *P<0.0001 versus wild type, Fisher's exact test. (C) The head region of L4 stage animals showing
des-2:GFP expression. In wild type,
des-2:GFP is expressed in the M1 head muscles and several neurons, including ALA and RID. In
ceh-17 and
ceh-14 null mutant L4 animals,
des-2:GFP is undetectable in ALA. Anterior is to the left, dorsal is up. WT, wild type.