Figure 4. Epistasis Analysis of Muscle Attachment Assembly(A) Localization of UNC-52/perlecan, PAT-3/β-integrin, UNC-112, PAT-4/ILK, DEB-1/vinculin, and UNC-89 in wild-type (1-6) and
pat-6(
st561) (1'-6') in 530 min embryos. Arrows in all panels except for 6' indicate body wall muscle attachments. Arrows in 6' indicate clumps of UNC-89 protein located in the cytoplasm of body wall muscle cells. All proteins visualized by antibody staining except for UNC-112, which was visualized using a functional
unc-112::gfp transgene.(B) Localization of PAT-6/actopaxin in wild-type,
unc-52(
st549),
pat-3(
st552),
unc-112(
gk1),
pat-4(
st551), and
deb-1(
st555) embryos (1-6). Arrows indicate regions of anti-PAT-6/actopaxin staining. PAT-6/actopaxin is properly recruited to muscle attachments in the wild-type (1) and
deb-1 (6) embryos, but not in the
unc-52(
st549),
pat-3(
st552),
unc-112(
gk1), or
pat-4(
st551) embryos (2-5). The embryos were also stained with monoclonal antibody MH27 to hypodermal cell junctions, which appear as a grid-like pattern and are indicated by arrowheads in panel 5. Scale bar (A1), 5 μm.