Figure 1. Expression of SMK-1 Is Coincident with DAF-16. (A-C) Using a C-terminal GFP-tagged SMK-1 under control of the endogenous
smk-1 promoter, nuclear GFP fluorescence is apparent in all intestinal cells (red arrows), head (B) and tail (C) neurons (open white arrows), and several hypodermal cells (closed white arrows).(D) SMK-1-GFP expression is absent in
smk-1 RNAi-treated animals. Endogenous gut autofluorescence remains. In (A)-(D), (a) is a fluorescent and (b) is a composite fluorescent/DIC image.(E)
smk-1 RNAi reduces SMK-1 protein level as indicated by the Western blot using affinity-purified anti-SMK-1 antibody.