C. elegans
rab-18 mutants display reduced lipid content under fed and fasted conditions as quantified by Nile Red (NR) and Oil Red O (ORO) staining: (A) Representative images of NR and ORO stained lipids in fixed wild type (N2) and
rab-18(
ok2020) mutant L4 stage larvae captured at 10X magnification. Scale bar: 100um. (B) Log2-fold change in NR fluorescence intensity of
rab-18 mutants compared to N2 worms under fed conditions. (C) Log2-fold change in ORO staining intensity of
rab-18 mutants compared to N2 worms under fed conditions. (D) Representative images of NR stained lipids in fixed wild type (N2) and
rab-18(
ok2020) mutant worms under fed and fasted (6 hours) conditions at 10X magnification. Scale bar: 100um. (E) Log2-fold change in NR fluorescence intensities of
rab-18 mutants compared to N2 worms under fed and fasted (6 hours) conditions. (F) Representative images of NR stained lipids in fixed wild type (N2),
rab-18(
ok2020), and
rab-18(
tm2121) mutant worms captured at 10X magnification. Scale bar: 100um. (G) Log2-fold change in NR fluorescence intensities of
rab-18(
ok2020) and
rab-18(
tm2121) mutants compared to N2 worms under fed conditions. Statistical analysis was performed with Student's t-test and with a one-way analysis of variance (ANOVA)****p<0.0001. Error bars represent S.D.