GFP (C) and b-galactosidase (D) expression, in the head region of transgenic adult C. elegans, driven by B0464.4 promoter fragments. The gfp reporter gene fusions were generated using MultiSite Gateway while the lacZ reporter gene fusions had been generated previously by conventional cloning. Expression is in nerve cells for B0464.4. Micro- graphs of C. elegans strains UL1111 (C) and UL42 (D) were captured at 400x magnification.
Figure 2. GFP (A,C) and b-galactosidase (B,D) expression, in the head region of transgenic adult C. elegans, driven by F44B9.2 (A,B) and B0464.4 (C,D) promoter fragments. The gfp reporter gene fusions were generated using MultiSite Gateway while the lacZ reporter gene fusions had been generated previously by conventional cloning. Expression is in body wall muscle cells for F44B9.2 and in nerve cells for B0464.4. Micro- graphs of C. elegans strains UL1110 (A), UL484 (B), UL1111 (C), and UL42 (D) were captured at 400x magnification.
GFP (A) and b-galactosidase (B) expression, in the head region of transgenic adult C. elegans, driven by F44B9.2 promoter fragments. The gfp reporter gene fusions were generated using MultiSite Gateway while the lacZ reporter gene fusions had been generated previously by conventional cloning. Expression is in body wall muscle cells for F44B9.2. Micrographs of C. elegans strains UL1110 (A) and UL484 (B) were captured at 400x magnification.
Fig. 2. Localisation of b-galactosidase activity in the pharynx of transgenic Caenorhabditis elegans during development. Staining of the pharyngeal metacorpus nuclei in adults (A), L3 larvae (B), L1 larvae (C) and eggs (D) is marked by the large arrowheads. Staining of additional nuclei within the terminal bulb of the pharynx is highlighted by the small arrowheads. Scale bar, 50 um.