Figure 1.
morc-1 Regulates Nuclear RNAi inthe Soma and Germline(A) Anti-FLAG immunofluorescence and DAPIstaining of worms expressing
morc-1::3xflag at theendogenous
morc-1 locus grown for two generations on empty vector or
morc-1 RNAi.(B)
morc-1(-) and
hrde-1(-) are germline mortal
at25C. Circles indicate the total number of viableprogeny of individual, self-fertilized worms at theindicated generation. Error bars indicate mean +-SD, n=10.(C)
morc-1(-) mutants are resistant to
lir-1 RNAi;this phenotype is rescued by expression of amorc-1::gfp extrachromosomal array (Ex+) drivenby the ubiquitous
dpy-30 promoter. Non-transgenic siblings are resistant to
lir-1 RNAi. Percentlarval arrest represents mean of two biologicalreplicates +- SD; n=85.(D)
morc-1() suppresses the sensitivity of
eri-1(-)to
lin-15b RNAi after two generations on lin-15bRNAi. Percent multivulva indicates mean of threebiological replicates +- SD; n=100.(E) Model for RNAi inheritance experiments. EVdenotes empty vector RNAi.(F)
morc-1(-) and
hrde-1(-) are defective forRNAi inheritance. On gfp RNAi, silencing of gfp iscompletely penetrant. Both
morc-1(-) and hrde1(-) fail to maintain gfp silencing in F1 and F2generations.(G) Quantification of percentage of animals withexpressed GFP in (F) showing mean of two biological replicates +- SD; n=100.See also Figure S1