Figure 6. INS-1:Venus Is Localized to the Synaptic Regions of AIA Interneurons. (A) Schematic diagram of AIA interneurons. (B) Expression pattern of Pins-1::Venus in AIA interneurons. (C and D) Expression pattern of Pins-1::
ins-1::Venus in AIA interneurons in wild-type N2 (C) and
unc-104(
e1265) (D) animals. In (B)-(D), stack images of various focal planes are merged. All panels show ventral view, anterior to the top. (E-G) Localization of INS-1::Venus and SNB- 1::mRFP in the nerve ring processes of AIA interneurons. The lateral view of
unc-13(
e51) adult animals expressing Pins-1::
ins-1:: Venus (green) and Pins-1::
snb-1::mRFP (magenda).
unc-13 mutants were used for better resolution of SNB-1::mRFP puncta (Chuang and Bargmann, 2005; Richmond et al., 1999). (H) INS-1::Venus rescues the learning defect of
ins-1(
nr2091) animals. Double asterisks represent significant differences from control
ins-1 animals carrying only the transformation marker (**p < 0.001 by Student's t test). Error bars represent SEM.