Figure 1. CHO-1:GFP localization in vivo and its UNC-104-dependent trafficking to cholinergic synapses. A, A CHO-1:GFP fusion protein localizes to punctate regions of cholinergic nerve processes and colabels with VAMP:mRFP1. Top panel, Transgenic C. elegans expressing a CHO-1:GFP fusion protein under the control of the endogenous
cho-1 promoter in a wild-type background (BY503). The arrows indicate en passant synapses of a cholinergic sublateral neuron. Bottom panels, Transgenic animals coexpressing CHO-1:GFP (BY503) and VAMP:mRFP1 (BY508) in a cholinergic sublateral neuron. Scale bars, 5 μm. B, CHO-1:GFP traffics on synaptic vesicles. Shown are head neurons expressing the CHO-1:GFP fusion protein in the wild-type (BY503) and
unc-104 (vtIs16;
unc-104(
e1265)) mutant backgrounds. The arrows indicate normal synaptic localization in the wild-type background and the loss of synaptic localization in the
unc-104 mutant background. The asterisks indicate where the fusion protein is trapped in the cell bodies of these neurons.