Figure 6. Several tissues express
hmgr-1 reporters. Expression of the Phmgr-1::GFP transcriptional reporter (A-B) and Phmgr-1::HMGR-1::GFP translational reporter (C - D). Structures labeled are as follows: ad (anal depressor), exc (excretory canal), gs (gonad sheath), hmc (head mesodermal cell), i (intestine),
pm1,
pm3 and
pm8 (pharyngeal muscles 1, 3 and 8), sp (spermatheca), vm (vulva muscles), vnc (ventral nerve cord). (E - F) and (G - H) respectively show GFP-negative and GFP-positive progeny from
hmgr-1(4368); Ex[Phmgr-1::HMGR-1::GFP
rol-6] transgenic animals grown on normalplates, i.e. without exogenous mevalonate; the GFP-positive progeny grow as well as wild-type animals while the GFP-negative progeny do not grow (I). ***p,0.001 using Student's t-test.