Figure 5. RT-PCR of the three
gar-1 mRNAs at various develop- mental stages. RT-PCR was performed, with a primer pair flanking the
i3 loop (MF-
i3/MR-
i3), using stage-specific RNA as a template (see Materials and Methods). The PCR products were analyzed on an 1% agarose gel. The expected sizes of the PCR products for
gar-1a,
gar-1b, and
gar-1c are 1281, 1188, and 1008 bp, respectively (from top to bottom). E, embryo; L1, first larval stage; L2, second larval stage; L3, third larval stage; L4, fourth larval stage; and A, young adult.