Picture from Tu, Shasha et al. (2023) MicroPubl Biol "Characterizing Three Azides for Their Potential Use as C. elegans ...."
Figure 1. KN3 as an alternative anesthetic for C elegans-related experiments:(A) Levamisole (Lev) and 1-Phenoxy-2-propanol (1P2P, 10%) are ineffective at paralyzing worms in chemotaxis assays to IAA. 1Lev, 1 mM levamisole; 10Lev, 10 mM levamisole. (B) All tested azides do not evoke chemotactic behavior. (C) IAA chemotaxis doesn't change using KN3 as an anesthetic, while it changes significantly using either TMSA or DPPA as an anesthetic. (D) 2-NN chemo-avoidance doesn't change using KN3 as an anesthetic, while it changes significantly using either TMSA or DPPA as an anesthetic. (E) Time courses showing the recovery of the worms in 18 minutes after the chemotaxis assays, statistical analysis was performed by comparing the area under the curve. (F) NaN3, KN3, TMSA, and DPPA all showed a certain extent of paralyzing effect in the buffer-based assay. Data in (A)-(D) and (F) are presented as box plots with all data points in magenta; the box plot displays the median, interquartile range, and minimal and maximal values. Data in (E) is presented as a line graph showing the median and interquartile range for the data at the indicated time point. Numbers above the x-axis denote the sample scale of each group; if the number is colored blue, the data is not normally distributed. vs NaN3 in (A) and (C)-(F); vs control in (B); *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.