Figure 2. C17ISO deficiency causes developmental arrest in early L1 independently from the DAF-2/DAF-16 food-sensing pathway. (A) The M-cell lineage during L1 development (data adapted from Sulston and Horvitz 1977). (B)
hlh-8::GFP expression in a single M cell in the C17ISO-deficient L1-arrested
elo-5(RNAi) larva. (C)
hlh-8::GFP expression in the two divided M cells in a wild-type L1 growing larva. (D) The changes in the seam cell patterns during L1 (adopted with permission from Blackwell Publishing [copyright 2002;
http://www.blackwell-synergy.com] from Knight et al. 2002). (E) AJM-1::GFP expression pattern at adherens junctions in a C17ISO-deficient L1-arrested
elo-5(RNAi) larva corresponds to a stage about 3 h post-hatching (Knight et al. 2002). (F) AJM-1::GFP expression pattern in a wild-type growing L1 animal corresponding to about 12 h post-hatching. (G) The disconnected seam cell shapes, typical of animals 6 h post-hatching (D), were observed in starved
daf-16(lf) mutants. (H) The seam cell shapes in
daf-16(lf);
elo-5(RNAi) are similar to that in
elo-5(RNAi) (E), and correspond to a stage about 3.3 h post-hatching (D).