Figure 2.
ceh-22b::VENUS Is Expressed Asymmetrically in the SGP Lineage. (A and B) Each row shows immunofluorescent (left), Nomarski (middle), and merged (right) images. Z1, anterior SGP; Z4, posterior SGP; Z1.a, distal Z1 daughter; Z1.p, proximal Z1 daughter. Images were taken at different exposures, so levels of expression are not comparable from stage to stage.(A)
ceh-22b::VENUS expression in hermaphrodites (XX) gonads. During L1, expression is detected in both SGPs, remains high in the distal SGP daughter (Z1.a), and is reduced in the proximal SGP daughter (Z1.p) (arrow). Expression is increased and maintained in the distal SGP daughter and its progeny, Z1.aa and Z1.ap, throughout L2, but disappears from the proximal SGP daughter and its descendants.(B)
ceh-22b::VENUS expression in male (XO) gonads. Expression was detected in the distal SGP daughters but is not visible in proximal SGP daughters at this exposure.(C) Summary of
ceh-22b::VENUS expression in L1 and L2 SGP lineage. Only Z1 lineage shown, although Z4 lineage was similar. Each vertical line represents a cell and each horizontal line represents a division. Black line, no detectable reporter expression; green line, reporter is expressed at level represented by line thickness (thin lines indicate low expression and thick lines indicate high expression); dotted line, diminished expression.(D)
ceh-22b::VENUS expression in nongonadal tissues. Top, expression in pharynx (bracket), head neuron (arrow), and ventral nerve cord (arrowheads). Middle, lower exposure to show distinct areas of pharyngeal expression. Bottom, expression in posterior intestinal cells.