Figure 5. The Recruitment of DYN-1::GFP to the Site of Engulfment Is Defective in
ced-1,
ced-6, and
ced-7 Mutants, but Not in
ced-5,
ced-10, or
ced-12 Mutants. Epifluorescence images of signals produced by embryos expressing Pced-1
ced-1::gfp, Pced-1
dyn-1::mrfp1, and Pced-1
dyn-1::gfp. Anterior is to the top. Ventral faces readers. The scale bar is 5 μm. All embryos are 330-380 min old.(A) Time-lapse images of (a-f) CED-1::GFP and (g-l) DYN-1::mRFP1 around the same cell corpse in a wild-type embryo. '0 min', the time point that CED-1::GFP is first detected on the budding pseudopods. Arrows indicate the extending pseudopods. One arrowhead indicates the engulfing cell. (m) Time to form the GFP or mRFP1 circles assayed in (a)-(l) and the duration of these circles.(B) (a) DIC and (b) GFP fluorescence images of the ventral surface of an embryo indicating the accumulation of DYN-1::GFP on the surface of three cell corpses (arrows) that are engulfed by three hypodermal cells (big arrowheads).(C) DYN-1::GFP accumulates to the persistent phagocytic cups (small arrowheads) and engulfed C3 cell corpse (arrows) (big arrowheads indicate the engulfing cell) in
ced-5(
n1812) and
ced-12(
n3261) mutant embryos, but not in
ced-1(
e1735) mutant embryos.(D) The DYN-1::GFP rings around engulfed C1 and C2 in different mutants (
ced-1(
e1735),
ced-5(
n1812),
ced-6(
n2095),
ced-7(
n1996),
ced-10(
n3246), and
ced-12(
n3261)). Arrows indicate the engulfed (a-g) C1 and (h-n) C2. The area around each arrow is amplified 1.8-fold and presented in the inset with the engulfing cell boundary highlighted. Images were captured 2-4 min after the full closure of the GFP circle.(E) Time-lapse images of the DYN-1::GFP circle around an engulfed C2 (arrows) in (a-g)
ced-5(
n1812) and (h-m)
ced-1(
e1735) embryos.