Figure 7. The Endosomal Marker HGRS-1 Clusters around Cell Corpses in a
dyn-1- and
ced-1-Dependent Manner. (A-F) All animals are wild-type, except for those shown in (E) and (F) (panels [b]-[e]). Transgenic reporters used are (A, B, and F) Pced-1
hgrs-1::gfp, (C) Pced-1
ced-1::gfp, (C and D) Pced-1
hgrs-1::mrfp1, and (D) Pced-1
dyn-1::gfp. The scale bars are 5 um. (A) HGRS-1::GFP in (a) a 200- to 230-min-old embryo, (b) a comma-stage embryo, and (c) the gonad of an adult hermaphrodite; (d), (e), and (f) are the GFP and DIC images of (a), (b), and (c), respectively. Arrows indicate (a) intestinal precursor cells and the (b and e) HGRS-1::GFP signal around one cell corpse. Arrowheads indicates the GFP signal in ventral hypodermal cells. Anterior is to the left. (B) (a) GFP and (b) GFP/DIC merged images of a comma-stage embryo. Arrows indicate cell corpses with a surrounding GFP signal. (c and d) 4-fold amplification to show the same two cell corpses found in (a) and (b) (arrows). Anterior is to the left. (C, D, and F) Epifluorescence images of 330- to 380-min-old embryos. Anterior is to the top. Ventral faces readers. (C) Coexpressed (a-g) CED-1::GFP and (h-n) HGRS-1::mRFP1 signals around cell corpse C2 (arrows) during and after it is engulfed by ABpraapppa (arrowheads). 0 min, the moment that pseudopods (visible with CED-1::GFP) start to extend around C2. The insets in (i)-(n) represent 2.6-fold amplified images of the region indicated by arrows. (o) The time needed to form GFP and mRFP1 circles around C2, as assayed in (a)-(n), and the duration of the circles. (D) Images of (a-d) DYN-1::GFP and (e-h) HGRS-1::mRFP around cell corpse C3 (arrows) at different times after it is engulfed by ABplaapppp (arrowheads). (E) The frequency that the HGRS-1::GFP circles are detected around C1 and C2 in different genetic backgrounds. n, the number of C1 and C2 analyzed. (F) HGRS-1::GFP signal in embryos of different genetic backgrounds. Arrows indicate the engulfed C1, C2, and C3.