Figure 3.
fil-1 and
fil-2 Encode Fasting-Induced Lipases Whose Expressions Are Dependent on
ire-1 and
hsp-4 (A) A phylogenic tree with FIL-1 and FIL-2. FIL-1 and FIL-2 are most similar to each other. C05D11.7 is another putative ATGL homolog in C. elegans, but was not induced by starvation in the present experiments (Table S2).(B) Relative amounts of
fil-1 and
fil-2 mRNA after 6 hr of fasting in
ire-1 and
hsp-4 RNAi worms. Quantitative real-time PCR was performed three times independently. The asterisks represent statistical significance (p < 0.05).(C) Fasting-induced
fil-1 and
fil-2 expression was independent of
xbp-1.
fil-1 and
fil-2 were upregulated by fasting even in the
xbp-1 (RNAi) background. (D) Expression patterns of FIL-1 and FIL-2 before and after fasting. FIL-1 and FIL-2 were induced by fasting only in the intestine. The inset in the lower right panel shows FIL-2 localization at a lipid droplet-like structure. The scale bar represents 25 um.