Figure 3. KIAA1018 Is Recruited to Sites of DNA Damage in C. elegans and Human Cells (A) C01G5.8 is a nuclear protein expressed in embryos and in the germline of C. elegans. C01G5.8::gfp was detected in the nucleus of embryos (A and B), mitotic germline zone (C and D), and late pachytene stage germ cells (E-J) of the transgenic opIs406[PC01G5.8::C01G5.8::gfp::
let-858(30UTR)] line. Cisplatin treatment resulted in relocalization of C01G5.8::gfp to distinct foci (arrows) (H-J). Scale bars, 10 mm. Human GFP-KIAA1018-WT colocalizes with FANCD2 (K) and RPA (L) to MMC-induced foci. U2OS cells stably expressing GFP-KIAA1018- WT were mock-treated or treated with MMC (120 ng/ml). After 20 hr, cells were stained with antibodies against FANCD2 (TexasRed) (K) or RPA (TexasRed) (L).