Fig. 5. The 1.3 kb cyclin B transcript appears specific to the paternal germline. In this northern blot, each lane contained 3 ug of poly(A)+ RNA. Lane 1, him-5
RNA; lane 2, staged L3/L4 RNA; lane 3, N2 mixed-stage (mostly adult) RNA. The probes used were: (A) the full-length C. elegans cyclin B cDNA; and (B) the full-length, 600 bp, C. elegansMSP cDNA (Burke and Ward, 1983). A pharyngeal myosin gene (Miller et al., 1986) was hybridized as a control to ensure equivalent loading of the RNA samples (not shown); for male RNA the vitellogenin probe used in Fig. 3 is not an appropriate control, as male worms do not produce vitellogenin (Schedin et al., 1991).