Figure 6. Expression of
lir-2::lacZ,
lir-1:lacZ,
lin-26::lacZ reporter constructs and expression of the C.b.LIN-26 protein. Embryos or larvae were fixed and stained with (A, E-J, O) polyclonal antibodies against b-galactosidase or (C, K, and L) C.e.LIN-26. (B and D) DAPI staining of the embryos shown in A and C, respectively. (M and N) Staining with the MH27 monoclonal antibody (mAb) of the embryos shown in K and L, respectively. Anterior is to the left. (A and B) A 50-cell stage
lir-2::lacZ (pML121) transgenic embryo; notice that the expression pattern is similar to that obtained by in situ hybridization. (C and D) A 50-cell stage C. briggsae embryos; antibodies against C.e.LIN-26 recognize a protein in C. briggsae embryos that is expressed in all cells, as
lir-2 is in C. elegans. (E) A 350-cell stage
lir-1::lacZ (pML169) transgenic embryo, dorsal view; expression is detected in hypodermal cells. (F and G) Comma stage
lir-1::lacZ (pML169) transgenic embryo, (F) lateral view or (G) central view; expression is detected in (F) hypodermal and support cells more weakly in most other cells, e.g. pharyngeal cells (open arrow and G) and intestinal cells (arrows). (H) A 350-cell stage
lir-1D-E::lacZ (pML165) transgenic embryo, dorsal view; expression is detected in hypodermal cells. (I) A 350-cell stage
lin-26::lacZ (pML20) transgenic embryo; expression is detected in hypodermal cells. (J) Comma stage
lin-26::lacZ (pML20) transgenic embryo; expression is detected in hypodermal and support cells, as can be seen with antibodies against C.e.LIN-26. (K and M) Wild-type C. elegans comma-stage embryo. (L and N) Wild-type C. briggsae embryo; the pattern is identical to that observed in C. elegans embryos, both with LIN-26 antiserum and the MH27 mAb, which detects a conserved epitope located at the adherens junctions separating hypodermal cells in both species. (O)
lir-1::lacZ (pML169) transgenic L4 larva, ventral view; expression is detected in most cells, except in the germline and the gut (n, neurons; ph, pharynx; m, body wall muscle; vc, ventral cord neuron; sg, somatic gonad; v, vulva). Note that because of mosaicism, few animals truly showed expression in all cells. Bar, 20 um (A-N); 50 um (O).