Figure 1.
sym-2(
mn617) neurodegeneration is rescued by expression of hrpa-1HsLCWT: A) After exposure to 22 hours of paraquat-induced oxidative stress,
sym-2(
mn617) causes a modest dye filling defect in the phasmid neurons asWTcompared to N2 animals, indicative of glutamatergic neurodegeneration. hrpa-1HsLC , but not D290V, empty, or
hrpa-1(
tm781), rescues this defect. One-way ANOVA F=51.28, p<0.0001 with p-values corrected for multiple comparisons of 0.0753 for WT v.
sym-2(
mn617), 0.1082 for
sym-2(
mn617) v. hrpa-1HsLCWT;
hrpa-1(
tm781);
sym-2(
mn617), and<0.0001 for hrpa-1HsLCD290V;
hrpa-1(
tm781) v. hrpa-1HsLCD290V;
hrpa-1(
tm781);
sym-2(
mn617). B) However, after exposure to only 4 hours of paraquat-induced oxidative stress,
sym-2(
mn617) does not cause a dye filling defect. One-way ANOVA F=8.353, p<0.0001 with p-values corrected for multiple comparisons of >0.9999 each for WT v.
sym-2(
mn617),
sym-2(
mn617) v. hrpa-1HsLCWT;
hrpa-1(
tm781);
sym-2(
mn617), and hrpa-1HsLCD290V;
hrpa-1(
tm781) v. hrpa-1HsLCD290V;
hrpa-1(
tm781);
sym-2(
mn617). WT are N2 animals; + are wild type progeny from tmC25[tmIs1241]/+ mothers. N=7-12 animals/genotype/trial, 3 trials. Mean with S.E.M. is reported, p-value on graph is from two-tailed t-test.