Figure 5. Expression Patterns of FBL-1 Proteins. Photos are oriented anterior to the left and dorsal to the top.(A and B) Fluorescence (left) and Nomarski (right) images of head (A) and tail (B) regions of wild-type L2 hermaphrodites expressing
fbl-1p::NLS::GFP. GFP was expressed in the intestine (arrows) and the body wall muscles (arrowhead).(C) Confocal (left) and Nomarski (right) images of a late L3 (C) wild-type hermaphrodite expressing
fbl-1C::Venus. FBL-1C-Venus localized to the surface of the gonads. The boundary of the gonad is depicted by a dotted line in the Nomarski image (also for [D]-[F]). Punctate fluorescence outside the gonads is of the FBL-1C-Venus in the Golgi apparatus of the gut cells or autofluorescence of the gut granules. The expression of
fbl-1C::Venus is especially strong in gut cells posterior to the gonad. The same is true in (D)-(F).(D and E) Confocal (left) and Nomarski (right) images of
fbl-1(
tk45) L4 hermaphrodites expressing
fbl-1C::Venus (D) and
fbl-1C::Venus
(k206) (E). The accumulation of FBL-1C-Venus is stronger than that of FBL-1C-Venus
(k206).(F) Confocal (left) and Nomarski (right) images of
fbl-1(
tk45);
mig-17(
k174) L4 hermaphrodites expressing
fbl-1C::Venus
(k206). FBL-1C-Venus
(k206) localizes to the surface of the gonads as in (E).(G and H) Confocal images of dissected gonads of
fbl-1(
tk45) hermaphrodites expressing
fbl-1C::Venus (G) and
fbl-1C::Venus
(k206) (H).(I) Confocal image of wild-type hermaphrodite with no transgene. The scale bars represent 20 μm. The scale bar in (C) is also applicable to (D)-(F), and the scale bar in (G) is also applicable to (H) and (I).(J and K) Western analysis of Venus fusion proteins with anti-GFP antibody.(J) Extracts from wild-type hermaphrodites with no transgene (lane 1),
fbl-1C::Venus (lane 2), or
fbl-1D::Venus (lane 3) were immunoblotted.(K) Extracts from
fbl-1(
tk45) hermaphrodites expressing
fbl-1C::Venus (lane 1) and
fbl-1C::Venus
(k206) (lane 2) were immunoblotted. α-tubulin was used as a loading control.