Figure 2. Perinuclear CEC-4 Localization Is Independent of H3K9 Methylation, and Varies from Tissue to Tissue(A) Single plane images of indicated embryo stages expressing CEC-4-mCh.(B) SR-SIM microscopy of CEC-4-mCh transgenic embryos, counterstained for nuclear pores, lamin or mCherry. Embryo sections and single nuclear planesshown.(C) Z-projection of CEC-4-mCh in
met-2 set-25 mutant background; images of mCherry alone and merged with gwIs4 GFP-LacI signal are shown. Insets: singleplain nuclei. Quantification of array distribution, n = foci scored.(D) Single plane confocal images of CEC-4- and EMR-1-mCh transgenic L1 larvae; scheme of L1 worm color-coded by tissue, M: muscle, I: intestine, H: hypoderm. Measured mCh signal intensity displayed as box plots in a.u. as in Figure 1. Black circles = outliers, n = number of nuclei per tissue; pair-wise comparisons for * and **p value < 0.001 in Wilcoxon test. Scale bar, 5 um in whole/section embryos and larvae; 2um in single nuclei/insets.See also Figure S2.