Figure 3.
tax-2 Protein Is Expressed in Sensory Neurons and Is Localized to Both the Sensory Cilia and the Axons. (A) GFP expression driven by the
tax-2::GFP construct, which includes the upstream sequences and the first intron (see Figure 2c). Left-lateral view of a transgenic worm showing the GFP-expressing AWC, AFD, ASE, ASG, ASJ, ADE, and BAG neurons. The GFP-expressing ASK, ASI, and AWB neurons are out of this plane of focus. A construct that contained only the upstream sequences drove GFP expression in the neurons AWC, AFD, ASE, ASI, ADE, BAG, and occasionally ASK (data not shown). All constructs were expressed occasionally in the tail sensory neuron PQR; a phasmid neuron (PHA or PHB) was also seen rarely. Anterior is to the left, and dorsal is up.(B) GFP expression driven by the tax-2Delta::GFP construct, which lacks the promoter and most of the first intron (mimicking the
tax-2(
p694) deletion; see Figure 2A). Left-lateral view of a transgenic worm showing the GFP-expressing neurons AWB, AWC, ASG, ASI, ASK, and ASJ. The positions of the sensory neurons are diagrammed at right. Anterior is to the left, and dorsal is up.(C) Localization of the Tax-2::GFP tagged protein. Left-lateral view of a transgenic animal expressing the GFP-tagged Tax-2 protein. Intense staining in the sensory cilia and axons is diagrammed in black, while weaker staining in the dendrites and cell bodies is diagrammed in gray. Anterior is at left, and dorsal is up.(D) Higher resolution view of Tax-2::GFP in the sensory cilia, generated by constrained iterative deconvolution. Dorsal view of the two bilaterally symmetrical AWC sensory cilia showing expression of the tagged Tax-2 protein. Other sensory cilia are out of the plane of focus. Anterior is at the top. Diagrammed dots indicate punctate staining in the dendrites. Scale bar, 5 μm.